DNA packaging serves all the purposes in an organism, except that it helps the DNA replicate easily. DNA packaging makes the DNA compact, thereby making it inaccessible for replication / transcription machinery. DNA needs to be unpackaged for efficient replication to occur within the cell.
CHAPTER 13 DNA Packaging in Chromatin and Chromosomes. Chromosomal DNA molecules of eukaryotes are thousands of times longer than the diameter of the nucleus and must therefore be highly compacted throughout the cell cycle. This folding is accomplished by combining the DNA with structural proteins to make chromatin.
Dna packaging mcq
A 3´→ 5´ proofreading exonuclease domain is intrinsic to most DNA polymerases. It allows the enzyme to check each nucleotide during DNA synthesis and excise mismatched nucleotides in the 3´ to 5´ direction. The proofreading domain also enables a polymerase to remove unpaired 3´ overhanging nucleotides to create blunt ends.
A) DNA amplified by PCR can be cloned. B) DNA is amplified at many points within a cellular genome. C) Newly synthesized DNA must be heat-denatured before the next round of DNA synthesis begins. D) The boundaries of the amplified DNA segment are determined by the synthetic oligonucleotides used to prime DNA synthesis.
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